4QU2
The structure of JMJD7 with alpha-KG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-28 |
Detector | NOIR-1 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.535, 59.516, 199.583 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.707 - 2.700 |
R-factor | 0.2131 |
Rwork | 0.208 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.812 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 21313 | |
Completeness [%] | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.1 M MES monohydrate, pH 6.0, 10% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |