4QTS
Crystal structure of Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.653, 96.319, 194.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.460 - 3.105 |
| R-factor | 0.2282 |
| Rwork | 0.225 |
| R-free | 0.28820 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.704 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.058 | 0.557 |
| Number of reflections | 25906 | |
| <I/σ(I)> | 15.1 | 2.7 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 50mM HEPES-Na (pH 6.8), 200mM potassium chrolide, 30% v/v Pentaerythritol propoxylate (5/4 PO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
