4QTK
Complex of WOPR domain of Wor1 in Candida albicans with the 17bp dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.713, 49.910, 84.307 |
| Unit cell angles | 90.00, 97.96, 90.00 |
Refinement procedure
| Resolution | 38.860 - 2.990 |
| R-factor | 0.2232 |
| Rwork | 0.221 |
| R-free | 0.27110 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 2.990 | 6.460 | 3.000 |
| Rmerge | 0.107 | 0.093 | 0.456 |
| Number of reflections | 12474 | ||
| <I/σ(I)> | 15.7 | ||
| Completeness [%] | 97.7 | 94.6 | 98.5 |
| Redundancy | 7.2 | 7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 289 | 0.10M Bis-Tris, 0.2M ammonnuim sulfate, 20% PEG 3350, pH 5.7, temperature 289K, VAPOR DIFFUSION, HANGING DROP |
