4QTK

Complex of WOPR domain of Wor1 in Candida albicans with the 17bp dsDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	75.713, 49.910, 84.307
Unit cell angles	90.00, 97.96, 90.00

Refinement procedure

Resolution	38.860 - 2.990
R-factor	0.2232
Rwork	0.221
R-free	0.27110
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	2.990	6.460	3.000
Rmerge	0.107	0.093	0.456
Number of reflections	12474
<I/σ(I)>	15.7
Completeness [%]	97.7	94.6	98.5
Redundancy	7.2	7	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	289	0.10M Bis-Tris, 0.2M ammonnuim sulfate, 20% PEG 3350, pH 5.7, temperature 289K, VAPOR DIFFUSION, HANGING DROP