4QTJ
Complex of WOPR domain of Wor1 in Candida albicans with the 13bp dsDNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 46.628, 46.628, 215.499 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.490 - 2.100 |
R-factor | 0.20803 |
Rwork | 0.206 |
R-free | 0.24834 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.819 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
Rmerge | 0.057 | 0.040 | 0.446 |
Number of reflections | 16046 | ||
<I/σ(I)> | 18.9 | ||
Completeness [%] | 95.2 | 86.6 | 94 |
Redundancy | 7.6 | 7.5 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 289 | 0.10M Bis-Tris, 0.2M ammonnuim sulfate, 20% PEG 3350, pH 5.7, temperature 289K, VAPOR DIFFUSION, HANGING DROP |