4QSL
Crystal Structure of Listeria Monocytogenes Pyruvate Carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-21 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 |
Unit cell lengths | 91.360, 132.615, 257.544 |
Unit cell angles | 86.65, 79.84, 70.07 |
Refinement procedure
Resolution | 47.160 - 3.280 |
R-factor | 0.23989 |
Rwork | 0.238 |
R-free | 0.28437 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bg5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.403 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.056 | 0.435 |
Number of reflections | 151836 | |
<I/σ(I)> | 11.3 | 1.6 |
Completeness [%] | 89.4 | 72.2 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 16% PEG 3350, 0.1M BIS-TRIS pH 6.5, 1% Tacsimate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |