4QSD
Crystal structure of atu4361 sugar transporter from Agrobacterium Fabrum C58, target efi-510558, with bound sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-05 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.025, 76.564, 67.873 |
Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.340 |
R-factor | 0.122 |
Rwork | 0.121 |
R-free | 0.15100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qsc |
RMSD bond length | 0.021 |
RMSD bond angle | 1.936 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.360 |
High resolution limit [Å] | 1.340 | 1.340 |
Rmerge | 0.037 | 0.460 |
Number of reflections | 80477 | |
<I/σ(I)> | 13.2 | 2.8 |
Completeness [%] | 97.9 | 52.2 |
Redundancy | 4.3 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 2.09 CRYSTALLIZATION CONDITIONS, PROTEIN: 10 MM BIS-TRIS, 500 MM NACL, 5% GLYCEROL, 5 MM DTT, TEV PROTEASE (1:100 RATIO); RESERVOIR: 0.1M TRIS-HCL, PH 8.5, 30% PEG400; CRYOPROTECTION: RESERVOIR PLUS 200MM SUCROSE; VAPOR DIFFUSION, SITTING DROP |