4QQW
Crystal structure of T. fusca Cas3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-28 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.789, 218.542, 123.749 |
Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
Resolution | 30.207 - 2.664 |
R-factor | 0.174 |
Rwork | 0.173 |
R-free | 0.22620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.410 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.058 | 0.378 |
Number of reflections | 115323 | |
<I/σ(I)> | 18.1 | 1.9 |
Completeness [%] | 92.7 | 86.8 |
Redundancy | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 100 mM MES, pH 6.5, and 5% 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |