4QQV

Extracellular domains of mouse IL-3 beta receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	197.150, 166.460, 128.000
Unit cell angles	90.00, 122.77, 90.00

Refinement procedure

Resolution	19.912 - 3.450
R-factor	0.2056
Rwork	0.203
R-free	0.24850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gys
RMSD bond length	0.012
RMSD bond angle	1.705
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1558))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.450	3.450
Rmerge	0.127
Number of reflections	45275
<I/σ(I)>	8.6
Completeness [%]	99.5
Redundancy	1.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	36% Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K