4QQK
Human HMT1 hnRNP methyltransferase-like protein 6 (S. cerevisiae) with GMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97912 |
| Spacegroup name | I 41 |
| Unit cell lengths | 95.211, 95.211, 108.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.520 - 1.880 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hc4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 71.520 | 50.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 5.100 | 1.880 |
| Rmerge | 0.060 | 0.027 | 0.704 |
| Number of reflections | 39024 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 38.2 | 8.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG 3350, 0.2 M KSCN, vapor diffusion hanging drop, temperature 291K |
