4QQI
Crystal structure of ANKRA2-RFX7 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-14 |
| Detector | R-Axis |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.459, 102.325, 32.566 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.030 |
| R-factor | 0.2213 |
| Rwork | 0.219 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3so8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.382 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.6) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.730 | 37.730 | 2.070 |
| High resolution limit [Å] | 2.020 | 9.040 | 2.020 |
| Rmerge | 0.177 | 0.010 | 0.010 |
| Total number of observations | 1313 | 7196 | |
| Number of reflections | 17145 | ||
| <I/σ(I)> | 10.5 | 34.7 | 2.1 |
| Completeness [%] | 99.5 | 98.3 | 93.8 |
| Redundancy | 6.9 | 5.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 291 | 0.1 M sodium hepes, 2.0 M ammonium sulfate, 2% PEG 400, pH 7.5, vapor diffusion, temperature 291K |
