4QQF
Crystal structure of mitochondrial import inner membrane translocase subunit TIM50
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 164.287, 164.287, 150.532 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.519 - 2.672 |
| R-factor | 0.2139 |
| Rwork | 0.212 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qle |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.584 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.168 | 2.770 |
| High resolution limit [Å] | 2.672 | 2.672 |
| Rmerge | 0.076 | 0.768 |
| Number of reflections | 58660 | |
| <I/σ(I)> | 42.2 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.0 M magnesium sulfate, 100 mM Tris, pH 7.5, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
