4QPZ
Crystal structure of the formolase FLS_v2 in space group P 21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 99.878, 136.564, 167.062 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 166.330 - 3.000 |
| R-factor | 0.21022 |
| Rwork | 0.208 |
| R-free | 0.25658 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ago |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 166.330 | 3.230 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.110 | 3.000 |
| Number of reflections | 89485 | ||
| <I/σ(I)> | 10.1 | 2.96 | 2.01 |
| Completeness [%] | 91.0 | 86 | 85.4 |
| Redundancy | 3.5 | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 15% PEG3350, 100 mM Succinic Acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
