4QPT
Structural Investigation of hnRNP L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.999987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.480, 37.970, 58.040 |
| Unit cell angles | 90.00, 105.35, 90.00 |
Refinement procedure
| Resolution | 31.421 - 1.351 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.20350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.526 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.440 | 1.380 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.059 | 0.558 |
| Number of reflections | 44418 | |
| <I/σ(I)> | 15 | 2.7 |
| Completeness [%] | 98.8 | 91.4 |
| Redundancy | 6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 25% MPD, 0.1M sodium chloride, 0.04M Sodium phosphate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
