4QPK

1.7 Angstrom Structure of a Bacterial Phosphotransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-02
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 43
Unit cell lengths	70.840, 70.840, 87.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.420 - 1.660
R-factor	0.165
Rwork	0.164
R-free	0.18780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fpp
RMSD bond length	0.007
RMSD bond angle	1.166
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1678))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.420
High resolution limit [Å]	1.660
Number of reflections	50679
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	292	0.2M Ammonium Sulfate, 0.1M Sodium Acetate pH 5.5, 10% (w/v) PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 292K