4QOJ
CRYSTAL STRUCTURE OF FMN QUINONE REDUCTASE 2 IN COMPLEX WITH RESVERATROL AT 1.85A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2014-02-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.960, 83.910, 106.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.090 - 1.850 |
| R-factor | 0.1801 |
| Rwork | 0.179 |
| R-free | 0.19790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qwx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.090 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 40334 | |
| <I/σ(I)> | 2.12 | |
| Completeness [%] | 91.3 | 84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1M HEPEs, 1.4M AmSO4, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
