4QO6
Structural studies of CdsD, a structural protein of the Type III secretion system (TTSS) of Chlamydia trachomatis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 106.600, 23.910, 118.650 |
Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
Resolution | 28.660 - 2.260 |
R-factor | 0.19696 |
Rwork | 0.195 |
R-free | 0.24133 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.766 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (AutoSolve) |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.660 | 2.390 |
High resolution limit [Å] | 2.260 | 2.260 |
Number of reflections | 13969 | |
Completeness [%] | 97.6 | 92.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 50 mM MES; pH 6.0 - 3 % PEG4000 - 15 % 2-propanol - 50 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |