4QNV
Crystal structure of Cx-SAM bound CmoB from E. coli in P6122
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.134, 82.134, 432.516 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.080 - 2.640 |
| R-factor | 0.22086 |
| Rwork | 0.218 |
| R-free | 0.26947 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.536 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.144 | 0.993 |
| Number of reflections | 26722 | |
| <I/σ(I)> | 15.5 | 4 |
| Completeness [%] | 99.2 | 100 |
| Redundancy | 8.5 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES:NaOH pH 7.5, 0.2M MgCl2, 30% (v/v) PEG400, 10mM TCEP:HCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
