4QN4
Crystal structure of Neuraminidase N6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 138.139, 138.139, 150.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.686 - 1.800 |
| R-factor | 0.1416 |
| Rwork | 0.141 |
| R-free | 0.15620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v0z |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.173 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 131314 | |
| Completeness [%] | 98.0 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 291 | 0.1 M sodium chloride, 0.1 M Tris, 8%(w/v) PEG 20000, pH 8.0, EVAPORATION, temperature 291K |
