4QMJ
The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.125, 79.125, 68.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.975 - 2.498 |
| R-factor | 0.2151 |
| Rwork | 0.208 |
| R-free | 0.27570 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.498 | 2.500 |
| <I/σ(I)> | 19.9 | 3.3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 7.4 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 10 mg/ml protein was added to an equal volume of a mother liquor containing 27.5% PEG 4000, 100 mM Tris pH 7.5, and 70 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
