4QMH
The XMAP215 family drives microtubule polymerization using a structurally diverse TOG array
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.12714, 0.97957 |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.788, 32.613, 59.795 |
| Unit cell angles | 100.05, 95.23, 109.95 |
Refinement procedure
| Resolution | 29.907 - 1.652 |
| R-factor | 0.1593 |
| Rwork | 0.157 |
| R-free | 0.18700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 25054 | |
| <I/σ(I)> | 33 | 5.7 |
| Completeness [%] | 95.3 | 88.9 |
| Redundancy | 1.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 15 mg/ml protein concentration, 21% PEG 4000, 100 mM Tris pH 8.5, and 400 mM Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
