4QMA

Crystal Structure of a Putative Cysteine Dioxygnase From Ralstonia eutropha: An Alternative Modeling of 2GM6 from JCSG Target 361076

Replaces:  2GM6

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.00000
Spacegroup name	P 41 21 2
Unit cell lengths	57.007, 57.007, 216.683
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.748 - 1.650
R-factor	0.1754
Rwork	0.174
R-free	0.20000
Starting model (for MR)	Fourier Synthesis based on 2GM6
RMSD bond length	0.014
RMSD bond angle	1.213
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.113	0.533
Number of reflections	44377
<I/σ(I)>	14.1	0.6
Completeness [%]	100.0	100
Redundancy	21	16.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	2.4M (NH4)2SO4, 0.1M TRIS, PH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	8	277	2.4M (NH4)2SO4, 0.1M TRIS, PH 8.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K