4QLN

structure of ydao riboswitch binding with c-di-dAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Spacegroup name	C 2 2 21
Unit cell lengths	77.874, 87.051, 144.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.250 - 2.650
R-factor	0.221
Rwork	0.217
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.820
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.650
Number of reflections	49287
Completeness [%]	1.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1			293	0.1 M Na-Bicine, pH 8.7-9.2 and 2.6-3.2 M (NH4)2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K