4QL7
Crystal structure of C-terminus truncated Alkylhydroperoxide Reductase subunit C (AhpC1-172) from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 137.129, 137.129, 145.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 3.750 |
R-factor | 0.274 |
Rwork | 0.273 |
R-free | 0.28980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o5r |
RMSD bond length | 0.004 |
RMSD bond angle | 0.747 |
Data reduction software | MOSFLM (3.3.20) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.950 |
High resolution limit [Å] | 3.750 | 3.750 |
Rmerge | 0.058 | 0.505 |
Number of reflections | 16581 | |
<I/σ(I)> | 14.6 | 3.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.8M ammonium sulfate, 100mM MES, pH 6.5, 10mM cobalt chloride, VAPOR DIFFUSION, HANGING DROP, temperature 291K |