4QL5
Crystal structure of translation initiation factor IF-1 from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12720 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.636, 83.636, 50.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.323 - 2.025 |
| R-factor | 0.1895 |
| Rwork | 0.189 |
| R-free | 0.20910 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.323 | 2.220 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.054 | 0.478 |
| Number of reflections | 12161 | |
| <I/σ(I)> | 19.4 | 3.4 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 8.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% polyethylene glycol (PEG) 3350 (w/v), 0.05 M zinc acetate dihydrate, cryoprotectant paratone oil, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
