4QL0
Crystal Structure Analysis of the Membrane Transporter FhaC (double mutant V169T, I176N)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.939280 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.380, 136.950, 110.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.950 - 2.500 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.25850 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 4qky |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.950 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 28332 | |
| Completeness [%] | 99.7 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 34% PEG 1000, 1% beta-octyl-glucoside, 500mM imidazole, 26mg/ml protein, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
