4QKW

Crystal structure of the zebrafish cavin4a HR1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.95370
Spacegroup name	C 1 2 1
Unit cell lengths	114.861, 31.961, 84.764
Unit cell angles	90.00, 94.46, 90.00

Refinement procedure

Resolution	42.254 - 1.700
R-factor	0.2139
Rwork	0.212
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.830
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.800	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.073	0.826
Number of reflections	211025
<I/σ(I)>	13	2.2
Completeness [%]	100.0	100
Redundancy	6.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	0.1M Hepes (pH 7.5), 20% PEG400, 30% 1,2 propanediol, VAPOR DIFFUSION, SITTING DROP, temperature 298K