4QKT
Azurin mutant M121EM44K with copper
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0750 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.285, 48.695, 95.068 |
| Unit cell angles | 90.00, 94.65, 90.00 |
Refinement procedure
| Resolution | 47.378 - 1.641 |
| R-factor | 0.1834 |
| Rwork | 0.181 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.983 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.378 |
| High resolution limit [Å] | 1.641 |
| Number of reflections | 59197 |
| Completeness [%] | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | pH8.0 100mM LiNO3 100 mM Tris 25%PEG4000 1:1 mixing-medium, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
