4QKL
Influenza A M2 wild type TM domain at high pH in the lipidic cubic phase under room temperature diffraction conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2013-09-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | I 4 |
| Unit cell lengths | 29.600, 29.600, 68.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.930 - 1.711 |
| R-factor | 0.1691 |
| Rwork | 0.166 |
| R-free | 0.19760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | chain A of 3C9J |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.606 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.150 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.158 | 0.470 |
| Number of reflections | 3128 | |
| <I/σ(I)> | 3.6 | 1.6 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LCP sandwich plates | 8 | 293 | 1.8 M calcium chloride, 0.9 M Tris HCl pH 8.0, 39.6% v/v PEG 400, 3% w/v xylitol additive, LCP sandwich plates, temperature 293K |
