4QKG
Monomeric form of human LLT1, a ligand for NKR-P1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9796 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 47.298, 47.298, 106.137 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.960 - 1.950 |
R-factor | 0.20138 |
Rwork | 0.194 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qkj |
RMSD bond length | 0.016 |
RMSD bond angle | 1.933 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.074 | 0.599 |
Number of reflections | 10555 | |
<I/σ(I)> | 21.2 | 2.9 |
Completeness [%] | 99.7 | 100 |
Redundancy | 6.3 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 294 | 2M ammonium sulfate, 0.1M sodium citrate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |