4QKC
Influenza A M2 wild type TM domain at low pH in the lipidic cubic phase under cryo diffraction conditions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.953724 |
| Spacegroup name | I 4 |
| Unit cell lengths | 29.310, 29.310, 67.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.064 - 1.100 |
| R-factor | 0.1097 |
| Rwork | 0.108 |
| R-free | 0.12760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | chain A of 3C9J |
| RMSD bond length | 0.043 |
| RMSD bond angle | 1.054 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.730 | 1.110 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.050 | 0.544 |
| Number of reflections | 11596 | |
| <I/σ(I)> | 17.3 | 3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LCP sandwich plates | 5.5 | 283 | 1.8 M calcium chloride, 0.9 M MES pH 5.5, 39.6% v/v PEG 400, 5% v/v Jeffamine M-600 pH 7.0 additive, LCP sandwich plates, temperature 283K |
