4QK9

Thermovirga lienii c-di-AMP riboswitch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-14
Detector	ADSC QUANTUM 1
Wavelength(s)	0.9792
Spacegroup name	P 21 3
Unit cell lengths	110.265, 110.265, 110.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.470 - 3.050
R-factor	0.1862
Rwork	0.182
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qk8
RMSD bond length	0.005
RMSD bond angle	0.987
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.110
High resolution limit [Å]	3.050	3.050
Number of reflections	9173
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6		0.5mM Spermine, 20mM Magnesium acetrate, 100mM Sodium achloride, 0.05M Sodium cacodylate pH6.5, 20%MPD, pH 6.0, VAPOR DIFFUSION, SITTING DROP