4QK8
Thermoanaerobacter pseudethanolicus c-di-AMP riboswitch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.105 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 114.868, 114.868, 114.709 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.717 - 3.050 |
R-factor | 0.1826 |
Rwork | 0.181 |
R-free | 0.19490 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.051 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.160 |
High resolution limit [Å] | 3.050 | 3.050 |
Number of reflections | 16997 | |
Completeness [%] | 99.7 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 1.5 M (NH4)2SO4, 0.1M HEPES, pH 7.3, and 0.2 M Li2SO4, VAPOR DIFFUSION, SITTING DROP |