4QK0
Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.383, 132.266, 109.045 |
| Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.258 |
| R-factor | 0.15965 |
| Rwork | 0.157 |
| R-free | 0.20048 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.645 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.290 |
| High resolution limit [Å] | 2.258 | 2.258 |
| Rmerge | 0.123 | |
| Number of reflections | 121797 | |
| <I/σ(I)> | 6.7 | 3.2 |
| Completeness [%] | 99.5 | 94.23 |
| Redundancy | 7.1 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG3350, 1% Tryptone, 0.1 M HEPES sodium, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
