4QJH

Crystal Structure of the Twister Ribozyme with the Nucleotide 5'- to the Cleavage Site Ordered at 4.1 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.1051
Spacegroup name	P 61 2 2
Unit cell lengths	228.770, 228.770, 101.060
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	198.120 - 3.880
R-factor	0.1873
Rwork	0.185
R-free	0.23224
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	1.349
Data reduction software	XDS
Data scaling software	XDS
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	198.121	50.000	4.260
High resolution limit [Å]	2.018	4.830	4.100
Rmerge	0.071		3.850
Number of reflections	12154
<I/σ(I)>	19		2
Completeness [%]	99.9	99.9	100
Redundancy	20.6		40.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	160 mM sodium citrate, pH 4.6, 700 mM ammonium sulfate, 1 M lithium sulfate, 3% pentaerythriotol ethoxylate (3/4 EO/OH), 3% 6-aminohexanoic acid, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	160 mM sodium citrate, pH 4.6, 700 mM ammonium sulfate, 1 M lithium sulfate, 3% pentaerythriotol ethoxylate (3/4 EO/OH), 3% 6-aminohexanoic acid, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	160 mM sodium citrate, pH 4.6, 700 mM ammonium sulfate, 1 M lithium sulfate, 3% pentaerythriotol ethoxylate (3/4 EO/OH), 3% 6-aminohexanoic acid, VAPOR DIFFUSION, SITTING DROP, temperature 298K