4QJF
X-ray crystal structure of Thermocuccus kodakarensis RNA polymerase Rpp4/Rpo7 (RpoF/RpoE) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-12 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 0.9714 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.465, 80.942, 97.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.794 - 2.312 |
R-factor | 0.1963 |
Rwork | 0.193 |
R-free | 0.26080 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.212 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: dev_1626)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 12575 |
Completeness [%] | 86.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | 0.1 M NaAcetate and 30 % glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 300K |