4QJ5
Structure of a fragment of human phospholipase C-beta3 delta472-581, bound to IP3 and in complex with Galphaq
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-04-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 205.918, 89.869, 93.261 |
Unit cell angles | 90.00, 101.80, 90.00 |
Refinement procedure
Resolution | 29.340 - 3.410 |
R-factor | 0.21502 |
Rwork | 0.212 |
R-free | 0.27671 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3OHM |
RMSD bond length | 0.005 |
RMSD bond angle | 1.016 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.8.0049) |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.142 | 0.440 |
Number of reflections | 22003 | |
<I/σ(I)> | 7.695 | 2.05 |
Completeness [%] | 96.1 | 95.9 |
Redundancy | 3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 277.15 | 100 mM MES, 200 mM NaCl, 5% (v/v) PEG 3350, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |