4QIQ

Crystal structure of D-xylose-proton symporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-03-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.127
Spacegroup name	P 31 2 1
Unit cell lengths	94.680, 94.680, 159.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.650 - 3.510
R-factor	0.308
Rwork	0.308
R-free	0.32400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gby
RMSD bond length	0.005
RMSD bond angle	0.782
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.0)
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	82.000
High resolution limit [Å]	3.500
Rmerge	0.087
Number of reflections	10580
<I/σ(I)>	5.9
Completeness [%]	97.7
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	8.5% PEG8000, 0.1M MES PH 5.6, 0.1M ZINC ACETATE, 30MM D-GLUCOSE, VAPOR DIFFUSION, HANGING DROP, temperature 277K