4QID
Crystal structure of Haloquadratum walsbyi bacteriorhodopsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-04-20 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.90000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.939, 29.799, 124.973 |
Unit cell angles | 90.00, 118.76, 90.00 |
Refinement procedure
Resolution | 28.870 - 2.570 |
R-factor | 0.20818 |
Rwork | 0.207 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.623 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.670 |
High resolution limit [Å] | 2.570 | 2.580 |
Rmerge | 0.168 | 0.791 |
Number of reflections | 48309 | |
<I/σ(I)> | 8.212 | 2.016 |
Completeness [%] | 97.8 | 98 |
Redundancy | 3.5 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPID CUBIC PHASE | 4 | 293 | ammonium sulfate, NaCl, sodium acetate, PEG 200, pH 4.0, LIPID CUBIC PHASE, temperature 293.0K |