4QI7
Cellobiose dehydrogenase from Neurospora crassa, NcCDH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-20 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 133.599, 141.851, 146.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.040 - 2.900 |
R-factor | 0.187 |
Rwork | 0.185 |
R-free | 0.23500 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.656 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1477)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 58.640 |
High resolution limit [Å] | 2.900 |
Number of reflections | 62494 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1 M Mes-OH pH 6.5, 1.5 M magnesium sulfate, 0.02 M lithium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |