4QI6

Cellobiose dehydrogenase from Myriococcum thermophilum, MtCDH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-01-26
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 43 21 2
Unit cell lengths	156.151, 156.151, 85.337
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.350 - 3.200
R-factor	0.221
Rwork	0.215
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	REFINED CYTOCHROM AND DEHYDROGENASE DOMAIN
RMSD bond length	0.006
RMSD bond angle	1.232
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1477))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.353
High resolution limit [Å]	3.200
Number of reflections	17929
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5	298	0.1 M ammonium sulfate, 0.4 M sodium formate, 30% (w/v) PEG monomethylether 5000, VAPOR DIFFUSION, SITTING DROP, temperature 298K