4QHY
The structure of aIF2gamma subunit H20F/D152A from archaeon Sulfolobus solfataricus complexed with GDPCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-14 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54179 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 187.689, 187.689, 187.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.969 - 3.290 |
| R-factor | 0.2288 |
| Rwork | 0.226 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nbs |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.701 |
| Data reduction software | PROTEUM2 |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1389)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.970 | 3.390 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.092 | 0.451 |
| Number of reflections | 16853 | |
| <I/σ(I)> | 10.89 | 1.87 |
| Completeness [%] | 99.8 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 301 | 4M sodium formiate, 0.1M sodium cacodylate, 1% MMEPEG 5K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 301K |
