4QHK
UCA (unbound) from CH103 Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.691, 71.148, 184.402 |
| Unit cell angles | 90.00, 93.81, 90.00 |
Refinement procedure
| Resolution | 46.846 - 3.487 |
| R-factor | 0.2696 |
| Rwork | 0.269 |
| R-free | 0.28490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jan |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.183 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
| High resolution limit [Å] | 3.487 | 9.480 | 3.487 |
| Rmerge | 0.144 | 0.054 | 0.373 |
| Number of reflections | 25281 | ||
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 96.5 | 93.7 | 95.1 |
| Redundancy | 3 | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM HEPES, pH 7.0, 3 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
