4QHF
Crystal structure of Methanocaldococcus jannaschii monomeric selecase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 51.260, 77.350, 31.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.700 - 2.100 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Dimeric selecase |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.700 | 42.700 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.070 |
Number of reflections | 7658 | |
<I/σ(I)> | 17.7 | 17.7 |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris-HCl, 0.2M sodium acetate, 30%(w/v) polyethylene glycol 4,000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |