4QH8
LC8 - Ana2 (237-246) Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07426 |
Spacegroup name | P 1 |
Unit cell lengths | 36.627, 44.802, 85.895 |
Unit cell angles | 99.33, 103.04, 91.79 |
Refinement procedure
Resolution | 35.594 - 1.900 |
R-factor | 0.188 |
Rwork | 0.185 |
R-free | 0.23580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pg1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.051 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 36096 | |
<I/σ(I)> | 19.5 | 7 |
Completeness [%] | 87.5 | 47.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | 0.19M ammonium acetate, 0.1M sodium acetate, 0.1% beta-mercaptoethanol, and 27% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |