4QH4
The GLIC pentameric Ligand-Gated Ion Channel (wild-type) crystallized in acetate buffer at pH3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2012-04-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 180.800, 133.383, 159.613 |
Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.260 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 60657 | |
Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 400 mM Sodium Isothiocyanate 100 mM Sodium acetate, 16% glycerol, 12-15% PEG4000, 2% DMSO, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |