4QH0
Crystal structure of NucA from Streptococcus agalactiae with magnesium ion bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-26 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 63 |
Unit cell lengths | 123.721, 123.721, 157.403 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.854 - 2.000 |
R-factor | 0.1575 |
Rwork | 0.155 |
R-free | 0.19030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qgo |
RMSD bond length | 0.004 |
RMSD bond angle | 0.816 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 89816 | |
<I/σ(I)> | 23.7 | 2.8 |
Completeness [%] | 97.2 | 79.9 |
Redundancy | 4.4 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 50mM Tris, 1.6M, ammonium sulfate, 10mM MgCl2 and 1:136 molar ratio of protein to 8mer duplex of 5'-GCGATCGC-3' DNA (not visible in structure), VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 7.5 |