4QGO
Crystal structure of NucA from Streptococcus agalactiae with no metal bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.591, 57.131, 62.422 |
| Unit cell angles | 109.40, 97.08, 90.16 |
Refinement procedure
| Resolution | 30.555 - 1.500 |
| R-factor | 0.154 |
| Rwork | 0.153 |
| R-free | 0.18030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3owa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 74966 | |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 96.3 | 91.4 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 1.6M ammonium sulfate, 0.1M sodium citrate and 0.2M NaCl, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
