4QFJ

The crystal structure of rat angiogenin-heparin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	39.415, 42.420, 154.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.189 - 2.196
R-factor	0.2195
Rwork	0.214
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bwk
RMSD bond length	0.010
RMSD bond angle	1.334
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.196	2.196
Number of reflections	13833
Completeness [%]	99.3	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	9% PEG8000, 5mM zinc acetate, 100mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, SITTING DROP, temperature 293K