4QFI

The crystal structure of rat angiogenin-heparin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97880
Spacegroup name	P 21 21 21
Unit cell lengths	43.039, 43.674, 154.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.302 - 1.784
R-factor	0.233
Rwork	0.232
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bwk
RMSD bond length	0.008
RMSD bond angle	1.058
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.780	1.780
Number of reflections	28081
Completeness [%]	98.7	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	24% PEG 8,000, 5mM zinc acetate, 100mM sodium cacodylate (pH 6.5), VAPOR DIFFUSION, SITTING DROP, temperature 293K