4QFF

Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, quadruple mutant, P212121 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-01-27
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	155.500, 156.520, 325.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.270 - 7.811
R-factor	0.2901
Rwork	0.288
R-free	0.33880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.437
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.273	91.273	8.010
High resolution limit [Å]	7.810	34.930	7.810
Rmerge	0.142	0.735	0.597
Number of reflections	8723	92	516
<I/σ(I)>	10.67	0.01	2.35
Completeness [%]	92.1	68.1	77.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	298	0.1 M sodium/potassium phosphate, pH 5.8, 10% PEG8000, 0.2 M sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K